Journal: Aging (Albany NY)

Article Title: The protective effects of Phoenixin-20 in tumor necrosis factor α (TNF-α)-induced cell senescence of rheumatoid arthritis fibroblast-like synoviocytes (FLS)

doi: 10.18632/aging.205024

Figure Lengend Snippet: Phoenixin-20 downregulated p53 and p21 in TNF-α-treated RA-FLSs. RA-FLSs were stimulated with TNF-α (10 ng/mL) with or without Phoenixin-20 (10, 20 nM) for 7 days. ( A ) mRNA of p53 and p21; ( B ) Protein of p53 and p21 ( ※ P < 0.01 vs. vehicle group; # , ## , P < 0.05, 0.01 vs. TNF-α group, N = 5–6).

Article Snippet: The primary antibodies against p53 (#AB_2860544, 1:2000, Sino Biological, China), p21 (1:1000, #ab109520, Abcam, USA), STAT6 (#ab32108, 1:1500, Abcam, USA), and β-actin (1:10000, #ab6276, Abcam, USA) were imported.

Techniques:

Journal: Aging (Albany NY)

Article Title: The protective effects of Phoenixin-20 in tumor necrosis factor α (TNF-α)-induced cell senescence of rheumatoid arthritis fibroblast-like synoviocytes (FLS)

doi: 10.18632/aging.205024

Figure Lengend Snippet: Overexpression of STAT6 abolished the protective effect of Phoenixin-20 on TNF-α-induced cellular senescence in RA-FLSs. Cells were transduced with lentiviral STAT6, followed by stimulation with TNF-α (10 ng/mL) and Phoenixin-20 (20 nM) for 7 days. ( A ) Western blot analysis revealed successful overexpression of STAT6; ( B ) mRNA of p53 and p21. ( C ) Cellular senescence was assayed using SA-β-gal staining ( ※ P < 0.01 vs. vehicle group; ## P < 0.01 vs. TNF-α group, * P < 0.05 vs. PNX-20 group, N = 5–6).

Article Snippet: The primary antibodies against p53 (#AB_2860544, 1:2000, Sino Biological, China), p21 (1:1000, #ab109520, Abcam, USA), STAT6 (#ab32108, 1:1500, Abcam, USA), and β-actin (1:10000, #ab6276, Abcam, USA) were imported.

Techniques: Over Expression, Transduction, Western Blot, Staining

Journal: Asian Pacific Journal of Cancer Prevention

Article Title: The Association between p53 Expression and Histopathology Grade of Astrocytoma

doi: 10.31557/apjcp.2025.26.7.2521

Figure Lengend Snippet: Figure 1. Immunohistochemistry Staining of p53 Mutant (High Power View, Magnification 400x). (A). Intense nuclear staining in immunopositive in ³10% of tumor cells was interpreted as positive (p53 mutant). (B). Weak nuclear staining was interpreted as negative as immunonegative (p53 wild-type)

Article Snippet: The research samples comprised Formalin-Fixed Paraffin-Embedded (FFPE) for Hematoxylin-Eosin (HE) and immunohistochemical staining with Anti-p53 mutant (M00001-3, Boster, Pleasanton, CA, USA).

Techniques: Immunohistochemistry, Staining, Mutagenesis

Journal: Oncology Letters

Article Title: Correlation of MMP-9 and p53 protein expression with prognosis in metastatic spinal tumor of lung cancer

doi: 10.3892/ol.2017.6887

Figure Lengend Snippet: H&E staining of p53 protein in the three experimental groups (from left to right: para-cancerous tissue, non-metastatic lung cancer tissue and lung cancer metastatic spinal tumor tissue). Samples obtained from para-cancerous tissue, non-metastatic lung cancer tissue and lung cancer metastatic spinal tumor tissue were stained using H&E staining method. There was p53 protein expression in para-cancerous tissue, increased expression in non-metastatic lung cancer tissues and abundant expression in metastatic spinal tumor tissue.

Article Snippet: Rabbit anti-human MMP-9 monoclonal antibody (Beijing Dingguo Changsheng Biotechnology Co. Ltd., Beijing, China), rabbit anti-human p53 monoclonal antibody (BD Biosciences, Franklin Lakes, NJ, USA), DAB chromogenic agent (Shanghai Haoran Biotechnology Co., Ltd., Shanghai, China), citrate buffer powder (Shanghai Haoran Biotechnology Co., Ltd.), hematoxylin (Shanghai Rongbai Biological Technology Co., Ltd., Shanghai, China) and eosin (Shanghai Rongbai Biotechnology Co., Ltd.) were used for immunohistochemistry.

Techniques: Staining, Expressing

Journal: Oncology Letters

Article Title: Correlation of MMP-9 and p53 protein expression with prognosis in metastatic spinal tumor of lung cancer

doi: 10.3892/ol.2017.6887

Figure Lengend Snippet: p53 protein expression in three tissue sample groups.

Article Snippet: Rabbit anti-human MMP-9 monoclonal antibody (Beijing Dingguo Changsheng Biotechnology Co. Ltd., Beijing, China), rabbit anti-human p53 monoclonal antibody (BD Biosciences, Franklin Lakes, NJ, USA), DAB chromogenic agent (Shanghai Haoran Biotechnology Co., Ltd., Shanghai, China), citrate buffer powder (Shanghai Haoran Biotechnology Co., Ltd.), hematoxylin (Shanghai Rongbai Biological Technology Co., Ltd., Shanghai, China) and eosin (Shanghai Rongbai Biotechnology Co., Ltd.) were used for immunohistochemistry.

Techniques: Expressing

Journal: Oncology Letters

Article Title: Correlation of MMP-9 and p53 protein expression with prognosis in metastatic spinal tumor of lung cancer

doi: 10.3892/ol.2017.6887

Figure Lengend Snippet: Correlation of MMP-9 and p53 protein expression in lung cancer metastatic spinal tumor tissues.

Article Snippet: Rabbit anti-human MMP-9 monoclonal antibody (Beijing Dingguo Changsheng Biotechnology Co. Ltd., Beijing, China), rabbit anti-human p53 monoclonal antibody (BD Biosciences, Franklin Lakes, NJ, USA), DAB chromogenic agent (Shanghai Haoran Biotechnology Co., Ltd., Shanghai, China), citrate buffer powder (Shanghai Haoran Biotechnology Co., Ltd.), hematoxylin (Shanghai Rongbai Biological Technology Co., Ltd., Shanghai, China) and eosin (Shanghai Rongbai Biotechnology Co., Ltd.) were used for immunohistochemistry.

Techniques: Expressing, Significance Assay

Journal: Oncology Letters

Article Title: Correlation of MMP-9 and p53 protein expression with prognosis in metastatic spinal tumor of lung cancer

doi: 10.3892/ol.2017.6887

Figure Lengend Snippet: Patient survival curves for MMP-9 and p53 protein expression positive and negative groups. (A) The survival rate of patients with negative p53 protein expression was significantly higher than that of patients with a positive p53 expression (χ 2 =9.826, p<0.01). (B) The survival rate of patients with a negative MMP-9 protein expression was higher than that of MMP-9 protein-positive patients (χ 2 =4.436, p<0.05). MMP-9, matrix metalloproteinase-9.

Article Snippet: Rabbit anti-human MMP-9 monoclonal antibody (Beijing Dingguo Changsheng Biotechnology Co. Ltd., Beijing, China), rabbit anti-human p53 monoclonal antibody (BD Biosciences, Franklin Lakes, NJ, USA), DAB chromogenic agent (Shanghai Haoran Biotechnology Co., Ltd., Shanghai, China), citrate buffer powder (Shanghai Haoran Biotechnology Co., Ltd.), hematoxylin (Shanghai Rongbai Biological Technology Co., Ltd., Shanghai, China) and eosin (Shanghai Rongbai Biotechnology Co., Ltd.) were used for immunohistochemistry.

Techniques: Expressing